Annexin V-FITC Kit
In early apoptosis, cells lose membrane phospholipid asymmetry, whilst the integrity of the cell membrane is maintained. The Annexin-V kits contain a viability dye to evaluate membrane integrity as well as annexin-V to assess membrane asymmetry.
In fact, phosphatidylserine (PS), a negatively charged phospholipid located in the inner surface of the plasma membrane, appears very early in apoptosis at the external cell surface. Annexin V, a calcium and phospholipid binding protein, binds preferentially to PS. The Annexin V kits employ recombinant annexin V labeled with biotin, fluorescein or R-phycoerythrin in together with the viability dyes propidium iodide (PI) or 7-amino actinomycin D (7-AAD).
Apoptotic cells are easily identified utilizing a rapid and simple staining procedure. Fixation of the cells is not necessary.
When cells are double-stained with annexin V and a viability dye, three different cell populations may be observed (see figure below): (i) live cells that do not stain either with annexin V or with the vital dye, (ii) necrotic or late apoptotic cells that stain with both annexin V and the vital dye, and (iii) early apoptotic cells that stain with annexin V only. Analysis may be performed on any flow cytometer equipped with a 488nm laser or Arc Lamp depending on the reagent kit chosen. Alternatively, the cells may be examined with a fluorescent microscope or cell imaging system equipped with the appropriate filters.
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PRODUCT AVAILABILITY AND REGULATORY STATUS DEPENDS ON COUNTRY REGISTRATION PER APPLICABLE REGULATIONS
The listed regulatory status for products correspond to one of the below:
IVD: In Vitro Diagnostic Products. These products are labeled "For In Vitro Diagnostic Use."
ASR: Analyte Specific Reagents. These reagents are labeled "Analyte Specific Reagents. Analytical and performance characteristics are not established."
CE: Products intended for in vitro diagnostic use and conforming to European Directive (98/79/EC). (Note: Devices may be CE marked to other directives than (98/79/EC)
RUO: Research Use Only. These products are labeled "For Research Use Only. Not for use in diagnostic procedures."
LUO: Laboratory Use Only. These products are labeled "For Laboratory Use Only."
No Regulatory Status: Non-Medical Device or non-regulated articles. Not for use in diagnostic or therapeutic procedures.